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pdest frt t0 flag brca1  (Addgene inc)
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Pdest Frt T0 Flag Brca1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flag brca1  (Addgene inc)
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Addgene inc flag brca1
Flag Brca1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pfastbac1 flag brca1 δexon11  (Addgene inc)
91
Addgene inc pfastbac1 flag brca1 δexon11
Plasmids used in this study.
Pfastbac1 Flag Brca1 δexon11, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcmv3-c-flag human brca1  (Sino Biological)
90
Sino Biological pcmv3-c-flag human brca1
Validation of <t>BRCA1</t> antibodies in western blot application with human cancer cell lines. (A) BRCA1 Abs test with MDA-MB-468 cells with/without siRNA transfection. (B) BRCA1 Ab test with U2OS cells with/without BRCA1 siRNA transfection. (C) BRCA1 Ab test with Hela inducible BRCA1 KO cell lines with/without IR.
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pcep4 expression plasmids for flag-tagged pit-1  (Kodak)
90
Kodak pcep4 expression plasmids for flag-tagged pit-1
Validation of <t>BRCA1</t> antibodies in western blot application with human cancer cell lines. (A) BRCA1 Abs test with MDA-MB-468 cells with/without siRNA transfection. (B) BRCA1 Ab test with U2OS cells with/without BRCA1 siRNA transfection. (C) BRCA1 Ab test with Hela inducible BRCA1 KO cell lines with/without IR.
Pcep4 Expression Plasmids For Flag Tagged Pit 1, supplied by Kodak, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Plasmids used in this study.

Journal: PLoS ONE

Article Title: Preparation and purification of mono-ubiquitinated proteins using Avi-tagged ubiquitin

doi: 10.1371/journal.pone.0229000

Figure Lengend Snippet: Plasmids used in this study.

Article Snippet: pFastbac1-FLAG-BRCA1 Δexon11 , Codon optimized human BRCA1 Δexon11 , Amp, Gent , gene synthesis by Gene Universal. Deposited Addgene: 137167 , a.

Techniques: Selection, Ubiquitin Proteomics

(A) Coomassie stained 4–12% Bis-Tris gel run in 1X MES buffer showing purified human FANCI:FANCD2, BRCA1 Δexon11 :BARD1, reconstituted nucleosome, PCNA, UBCH5C (S22R), Avi-ubiquitin, FANCB-FANCL-FAAP100 (BL100), FANCC-FANCE-FANCF (CEF), UBE1 and UBE2T (lanes 2–11). (B) Western blot of the time course ubiquitination reaction of human FANCI:FANCD2 at 25 °C, 30 °C and 37 °C. The percentage of mono-ubiquitinated FANCD2 or FANCI were calculated and showed under each western blot panel. (C) PCNA mono-ubiquitination time course experiments using UBCH5C as an E2 enzyme. (D) Western blots of time course experiments revealing that BRCA1 Δexon11 and BARD1 were auto-ubiquitinated at multiple lysine residues in the presence of UBCH5C. (E) Mono-ubiquitination timecourse of nucleosomes as substrates in the presence of BRCA1 Δexon11 :BARD1 and UBCH5C. Experiments in (B-E) were performed in triplicate with similar results.

Journal: PLoS ONE

Article Title: Preparation and purification of mono-ubiquitinated proteins using Avi-tagged ubiquitin

doi: 10.1371/journal.pone.0229000

Figure Lengend Snippet: (A) Coomassie stained 4–12% Bis-Tris gel run in 1X MES buffer showing purified human FANCI:FANCD2, BRCA1 Δexon11 :BARD1, reconstituted nucleosome, PCNA, UBCH5C (S22R), Avi-ubiquitin, FANCB-FANCL-FAAP100 (BL100), FANCC-FANCE-FANCF (CEF), UBE1 and UBE2T (lanes 2–11). (B) Western blot of the time course ubiquitination reaction of human FANCI:FANCD2 at 25 °C, 30 °C and 37 °C. The percentage of mono-ubiquitinated FANCD2 or FANCI were calculated and showed under each western blot panel. (C) PCNA mono-ubiquitination time course experiments using UBCH5C as an E2 enzyme. (D) Western blots of time course experiments revealing that BRCA1 Δexon11 and BARD1 were auto-ubiquitinated at multiple lysine residues in the presence of UBCH5C. (E) Mono-ubiquitination timecourse of nucleosomes as substrates in the presence of BRCA1 Δexon11 :BARD1 and UBCH5C. Experiments in (B-E) were performed in triplicate with similar results.

Article Snippet: pFastbac1-FLAG-BRCA1 Δexon11 , Codon optimized human BRCA1 Δexon11 , Amp, Gent , gene synthesis by Gene Universal. Deposited Addgene: 137167 , a.

Techniques: Staining, Purification, Ubiquitin Proteomics, Western Blot

Validation of BRCA1 antibodies in western blot application with human cancer cell lines. (A) BRCA1 Abs test with MDA-MB-468 cells with/without siRNA transfection. (B) BRCA1 Ab test with U2OS cells with/without BRCA1 siRNA transfection. (C) BRCA1 Ab test with Hela inducible BRCA1 KO cell lines with/without IR.

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Validation of BRCA1 antibodies in western blot application with human cancer cell lines. (A) BRCA1 Abs test with MDA-MB-468 cells with/without siRNA transfection. (B) BRCA1 Ab test with U2OS cells with/without BRCA1 siRNA transfection. (C) BRCA1 Ab test with Hela inducible BRCA1 KO cell lines with/without IR.

Article Snippet: The plasmid pCMV3-C-Flag human BRCA1 was purchased from Sino Biological Inc. (Beijing, China).

Techniques: Western Blot, Transfection

Analysis of BRCA1 antibodies by immunoprecipitation in human cell lines. (A) BRCA1 IPs with 293T/3XFlag-hBRCA1 stably transfected cell lysate. (B) BRCA1 IPs with U2OS/3xFLAG-APEX2-fused BRCA1 cell lysate. (C) BRCA1 IPs with MDA-MB-468 cell lysate.

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Analysis of BRCA1 antibodies by immunoprecipitation in human cell lines. (A) BRCA1 IPs with 293T/3XFlag-hBRCA1 stably transfected cell lysate. (B) BRCA1 IPs with U2OS/3xFLAG-APEX2-fused BRCA1 cell lysate. (C) BRCA1 IPs with MDA-MB-468 cell lysate.

Article Snippet: The plasmid pCMV3-C-Flag human BRCA1 was purchased from Sino Biological Inc. (Beijing, China).

Techniques: Immunoprecipitation, Stable Transfection, Transfection

Analysis of BRCA1 antibodies by immunofluorescence in human U2OS cells. Laser stripes of BRCA1 and γH2AX at 1h time point after laser micro-irradiation in U2OS cells.

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Analysis of BRCA1 antibodies by immunofluorescence in human U2OS cells. Laser stripes of BRCA1 and γH2AX at 1h time point after laser micro-irradiation in U2OS cells.

Article Snippet: The plasmid pCMV3-C-Flag human BRCA1 was purchased from Sino Biological Inc. (Beijing, China).

Techniques: Immunofluorescence, Irradiation

Analysis of BRCA1 antibodies by ChIP-qPCR in human cancer cells. (A) BRCA1 immunoblots with CTT20 (inducible Cas9 in HeLa), A9.2 and A10.2 (two BRCA1 inducible KO cell lines) cells with/without doxycycline treatment. (B-F) ChIP-qPCR profiles of CTT20 (inducible Cas9 in HeLa), A9.2 and A10.2 cells with/without doxycycline treatment using BRCA1(Ab1) antibody at the BRCA1 (B), ATRIP (C), EXO1 (D), MAD2L1 (E) and PPM1D (F) promoter regions. 6 μg of Ab (if concentration of Ab is provided) or 15 μl of serum (if concentration is not provided) is used. **p < 0.01, *p < 0.05, Student's t-test.

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Analysis of BRCA1 antibodies by ChIP-qPCR in human cancer cells. (A) BRCA1 immunoblots with CTT20 (inducible Cas9 in HeLa), A9.2 and A10.2 (two BRCA1 inducible KO cell lines) cells with/without doxycycline treatment. (B-F) ChIP-qPCR profiles of CTT20 (inducible Cas9 in HeLa), A9.2 and A10.2 cells with/without doxycycline treatment using BRCA1(Ab1) antibody at the BRCA1 (B), ATRIP (C), EXO1 (D), MAD2L1 (E) and PPM1D (F) promoter regions. 6 μg of Ab (if concentration of Ab is provided) or 15 μl of serum (if concentration is not provided) is used. **p < 0.01, *p < 0.05, Student's t-test.

Article Snippet: The plasmid pCMV3-C-Flag human BRCA1 was purchased from Sino Biological Inc. (Beijing, China).

Techniques: Western Blot, Concentration Assay

Validation of BRCA1 antibodies in Western blot with mouse testis and MEC cells. (A) A representative 8-week-old BRCA1 Δ11/Δ11 mouse on the right was smaller compared with its WT littermate on the left in the p53+/- background. (B) The spleen and testis isolated from BRCA1 Δ11/Δ11 and WT littermate male mice at 2-month old. (C) BRCA1 WB with testis tissue lysate from BRCA1 Δ11/Δ11 and WT littermate male mice at 2-month old. (D) BRCA1 WB with primary mouse mammary epithelial cell lysate from BRCA1 Δ11/Δ11 and WT littermate female mice at 6-week old.

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Validation of BRCA1 antibodies in Western blot with mouse testis and MEC cells. (A) A representative 8-week-old BRCA1 Δ11/Δ11 mouse on the right was smaller compared with its WT littermate on the left in the p53+/- background. (B) The spleen and testis isolated from BRCA1 Δ11/Δ11 and WT littermate male mice at 2-month old. (C) BRCA1 WB with testis tissue lysate from BRCA1 Δ11/Δ11 and WT littermate male mice at 2-month old. (D) BRCA1 WB with primary mouse mammary epithelial cell lysate from BRCA1 Δ11/Δ11 and WT littermate female mice at 6-week old.

Article Snippet: The plasmid pCMV3-C-Flag human BRCA1 was purchased from Sino Biological Inc. (Beijing, China).

Techniques: Western Blot, Isolation

Validation of BRCA1 antibodies in MEF cells. (A) MEF cell lysates were prepared and immunoprecipitation was performed using the indicated BRCA1 antibodies pre-coupled to protein A/G beads. (B) Summary of BRCA1 specific peptides detected by MS in IP samples with two anti-BRCA1 antibodies.

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Validation of BRCA1 antibodies in MEF cells. (A) MEF cell lysates were prepared and immunoprecipitation was performed using the indicated BRCA1 antibodies pre-coupled to protein A/G beads. (B) Summary of BRCA1 specific peptides detected by MS in IP samples with two anti-BRCA1 antibodies.

Article Snippet: The plasmid pCMV3-C-Flag human BRCA1 was purchased from Sino Biological Inc. (Beijing, China).

Techniques: Immunoprecipitation

Analysis of BRCA1 antibodies by immunofluorescence in MEF cells. Laser stripes of BRCA1 and γH2AX at 1h time point after laser micro-irradiation in MEF cells.

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Analysis of BRCA1 antibodies by immunofluorescence in MEF cells. Laser stripes of BRCA1 and γH2AX at 1h time point after laser micro-irradiation in MEF cells.

Article Snippet: The plasmid pCMV3-C-Flag human BRCA1 was purchased from Sino Biological Inc. (Beijing, China).

Techniques: Immunofluorescence, Irradiation

Antibodies used in this study

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Antibodies used in this study

Article Snippet: The plasmid pCMV3-C-Flag human BRCA1 was purchased from Sino Biological Inc. (Beijing, China).

Techniques: