human brca1 gene orf cdna clone expression plasmid,c-flag tag (Sino Biological)

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Full length Clone DNA of Human BRCA1, DNA repair associated with C terminal Flag tag.

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Addgene inc addgene 52504 plasmids
Addgene 52504 Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+brca1/pDEST+3x+Flag-pcDNA5-FRT%2FT0-BRCA1+(Plasmid+%2352504)/bio_rxiv__2024__02__11__579865-108-37-37
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pdest flag brca1 (Addgene inc)

Addgene inc pdest flag brca1
Pdest Flag Brca1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+brca1/pDEST-FRT%2FT0-Flag-BRCA1+(Plasmid+%2371117)/pmc06847465-30-6-7
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pfastbac1 flag brca1 δexon11 (Addgene inc)

Addgene inc pfastbac1 flag brca1 δexon11

Pfastbac1 Flag Brca1 δexon11, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pfb 5' flag brca1 (1863) (plasmid #14507) (Addgene inc)

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Standard format: Plasmid sent in bacteria as agar stab

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pdest-frt/t0-flag-brca1 (plasmid #71104) (Addgene inc)

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Standard format: Plasmid sent in bacteria as agar stab

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human brca1 gene orf cdna clone expression plasmid, n-flag tag (Sino Biological)

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Full length Clone DNA of Human BRCA1, DNA repair associated

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Journal: PLoS ONE

Article Title: Preparation and purification of mono-ubiquitinated proteins using Avi-tagged ubiquitin

doi: 10.1371/journal.pone.0229000

Figure Lengend Snippet: Plasmids used in this study.

Article Snippet: pFastbac1-FLAG-BRCA1 Δexon11 , Codon optimized human BRCA1 Δexon11 , Amp, Gent , gene synthesis by Gene Universal. Deposited Addgene: 137167 , a.

Techniques: Selection, Ubiquitin Proteomics

(A) Coomassie stained 4–12% Bis-Tris gel run in 1X MES buffer showing purified human FANCI:FANCD2, BRCA1 Δexon11 :BARD1, reconstituted nucleosome, PCNA, UBCH5C (S22R), Avi-ubiquitin, FANCB-FANCL-FAAP100 (BL100), FANCC-FANCE-FANCF (CEF), UBE1 and UBE2T (lanes 2–11). (B) Western blot of the time course ubiquitination reaction of human FANCI:FANCD2 at 25 °C, 30 °C and 37 °C. The percentage of mono-ubiquitinated FANCD2 or FANCI were calculated and showed under each western blot panel. (C) PCNA mono-ubiquitination time course experiments using UBCH5C as an E2 enzyme. (D) Western blots of time course experiments revealing that BRCA1 Δexon11 and BARD1 were auto-ubiquitinated at multiple lysine residues in the presence of UBCH5C. (E) Mono-ubiquitination timecourse of nucleosomes as substrates in the presence of BRCA1 Δexon11 :BARD1 and UBCH5C. Experiments in (B-E) were performed in triplicate with similar results.

Journal: PLoS ONE

Article Title: Preparation and purification of mono-ubiquitinated proteins using Avi-tagged ubiquitin

doi: 10.1371/journal.pone.0229000

Figure Lengend Snippet: (A) Coomassie stained 4–12% Bis-Tris gel run in 1X MES buffer showing purified human FANCI:FANCD2, BRCA1 Δexon11 :BARD1, reconstituted nucleosome, PCNA, UBCH5C (S22R), Avi-ubiquitin, FANCB-FANCL-FAAP100 (BL100), FANCC-FANCE-FANCF (CEF), UBE1 and UBE2T (lanes 2–11). (B) Western blot of the time course ubiquitination reaction of human FANCI:FANCD2 at 25 °C, 30 °C and 37 °C. The percentage of mono-ubiquitinated FANCD2 or FANCI were calculated and showed under each western blot panel. (C) PCNA mono-ubiquitination time course experiments using UBCH5C as an E2 enzyme. (D) Western blots of time course experiments revealing that BRCA1 Δexon11 and BARD1 were auto-ubiquitinated at multiple lysine residues in the presence of UBCH5C. (E) Mono-ubiquitination timecourse of nucleosomes as substrates in the presence of BRCA1 Δexon11 :BARD1 and UBCH5C. Experiments in (B-E) were performed in triplicate with similar results.

Article Snippet: pFastbac1-FLAG-BRCA1 Δexon11 , Codon optimized human BRCA1 Δexon11 , Amp, Gent , gene synthesis by Gene Universal. Deposited Addgene: 137167 , a.

Techniques: Staining, Purification, Ubiquitin Proteomics, Western Blot

flag brca1 Search Results

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